Factors To Consider When Buying ELISA Test Kits

Factors To Consider When Buying ELISA Test Kits

by Annabelle Holman

To determine if antigens and antibodies exist in a specific sample you need a biochemistry procedure referred to as Enzyme linked immunosorbent assay. The procedure has been in use for many years in the field of pathology and medicine. <A href="http://www.elisatestkits.com">ELISA test kits</A> are used for allergies, diabetes, steroids, cancer, parasitology, thyroid, fertility, autoimmune diseases and infectious diseases.

There are features that you should look for when buying ELISA kits. The first thing is the assay format. You need to ensure that you get the right format for your experiment. It is also important to ensure that you get the right container system. Normally ELISAs are done in 384 or 96 well plates but there are several other formats.

One of the most common formats is referred to as sandwich assay. It is named so because there are two different antibodies that sandwich the antigen. This format is popular because it is robust and sensitive. There are many times when this format is not appropriate especially if the antigen is small. It is difficult to attach antibodies which are bulky to a small antigen.

The sort of antibodies you working with are likewise put into attention. In ELISAs you can utilize both polyclonal and monoclonal antibodies. There are cases that call for a consolidation of both. At the point when picking antibodies there are a few variables to place into consideration. For detection it is fitting to use monoclonal antibodies. Polyclonal antibodies can be used for capturing. In the event that you take after this tip you ought to have the capacity to catch all antigens and afterward utilize monoclonal antibodies to distinguish some particular antigens.

You need to make sure that the capture and detection antibodies are matching. The two should not compete for the same site on the antigen. Instead they should recognize varying epitopes on the antigen. Your results will be skewed if you do not ensure there is no interference. There are ways of ensuring this does not happen. The most recommended way is to get a pair that is already matched from the supplier. You can use the internet to find antibody suppliers.

Another factor to consider is interference and cross reactivity. If antibodies on the animal are compatible this could cause cross reactivity. It is easy to get antibodies for the animal that you are working with. You should always note if the cross reactivity might cause any issues. When you are working with the sandwich format it is important to match the primary detection antibodies with the secondary ones that are used for detection.

You ought to figure out the washing and in addition the blocking buffers. Now and again you may discover compounds that will influence collaboration of the antibodies and antigens. You ought to consider detection method too. There are a couple of methods used for binding. Discover an pack that has the sort of detection method you would like.

If you want to know more about these features you can request details from representatives from the companies that sell the kits. They will be glad to inform you more about them and how to use them. The internet is also a source of information.



When you need a company that offers <a href="http://www.elisatestkits.com">ELISA test kits</a> we would recommend that you look at this website for your needs. Have a look at the online store by clicking on this link http://www.elisatestkits.com.

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